Job Description

Job id: 073175. Salary: \xc2\xa332,179 - \xc2\xa335,596 per annum, including London Weighting Allowance.

Posted: 15 August 2023. Closing date: 20 August 2023.

Business unit: Faculty of Life Sciences & Medicine. Department: Comprehensive Cancer Centre.

Contact details: Prof. Eric So. Eric.so@kcl.ac.uk

Location: Denmark Hill. Category: Professional & Support Services.

Job description

The purpose of this post is to develop and expedite projects aimed at characterizing leukemia networks in leukemia using newly developed disease models in the lab. Transcription factors and their associated epigenetic modifying enzymes are frequently deregulated or even mutated in human acute myeloid leukemia (Zeisig et al., 2012, Cancer Cell). Over the years, our lab has focused on characterization of key transcriptional/epigenetic networks and their emerging crosstalk with DNA damage repair (DDR) mechanisms critical for leukemia development (Kwok et al., 2006, Cancer Cell; Zeisig et al., 2007, Cancer Cell; Cheung et al., 2007, Nat Cell Biology; Arteaga et al., 2013, Cancer Cell; Esposito et al., 2015, Nature Medicine; Cheung et al., 2016, Cancer Cell; Luo et al., 2019, Cancer Cell; Zeisig et al., 2021, Science Translational Medicine; Luo et al., 2022, Molecular Cell,). Details about the lab can be found at . Candidates are desirable to have background in areas of DDR, epigenetic gene regulation and/or cancer biology.

This post is aiming to assist Professor So in the design and expediting of his research projects for publication of academic papers and securing of grants.

The post holder will:

To help to maintain the general operation of the lab.

To introduce appropriate new techniques and procedures, ensuring that all new techniques and procedures techniques are adequately validated and documented and ensure that colleagues are trained in new techniques and procedures.

To directly participate research projects.

This post will be offered on an a fixed-term contract for 24 months, with possibility for renewal.

This is a full-time post - 100% full time equivalent

Key responsibilities

• FACS sorting and analysis
• Tissue culture and cell transformation assay
• In vivo disease modelling using genetically modified mice
• working and characterizing of human patient samples
• Immunofluorescent staining
• Leukemia and stem cell biology studies

The above list of responsibilities may not be exhaustive, and the post holder will be required to undertake such tasks and responsibilities as may reasonably be expected within the scope and grading of the post.

Skills, knowledge, and experience

• Providing assistance on a day-to-day basis according to the ever-changing needs of the Group as directed by the Head of the Leukaemia and Stem Cell Biology Group. This will require a commitment to a very flexible and service-oriented / team-working approach.
• Be trained in procedures as required by more senior and experienced members of the Group.
• Assist with the training of new members of staff and students in the routine procedures of the Group.
• Develop and improve their own scientific knowledge and awareness of developments within the field of leukaemia stem cell biology via attendance of relevant meetings and conferences.

Essential criteria

1. BSc Hons in a biomedical sciences subject or equivalent (eg biochemistry)

2. A thorough knowledge of cell and molecular biology

3. Proficiency in performing experimental procedures with animal (mouse).

4. Competent in the interpretation and technical validation of results

5. Good verbal and written communication skills in English

6. Ability to think logically, adapt to changing requirements and use initiative

7. Ability to work to deadlines under pressure

8. Ability to work as part of a team and independently

9. Ability to organize workload and to respond accordingly to changes in the workload (e.g. be flexible to work outside normal working hour)

10. Experience of work with biological samples, laboratory information systems

Desirable criteria

1. Experiences in handling mouse or maintenance of colony

2. Master or a higher degree in which investigations of epigenetic regulation/DDR/Biochemistry formed a substantial part

3. Relevant publication in peer-reviewed journal

4. Experience in the study of transcriptional / epigenetic processes

5. Experience of procedures used in the characterisation of haemopoiesis, including xenotransplantation models

King\'s College London